Part:BBa_K2333005:Design

mf-Lon Protein Degradation Tag E (medium-weak)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This specific sequence was created by E. coli codon optimizing the amino acid sequence provided in the supplement by Collins et al, as a DNA sequence was not provided.

Source

This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT.

We use the website www.jcat.de to optimize the codon sequence for E. coli.

References

[1] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281.

[2] Gur E, Sauer RT. Evolution of the ssrA degradation tag in Mycoplasma: Specificity switch to a different protease. Proceedings of the National Academy of Sciences. 2008;105(42):16113–16118.